Assessment of folic acid and DNA damage in cleft lip and cleft palate

  • Sivakumar Brooklyin Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India.
  • Rashmoni Jana | drrashmoni@gmail.com Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India.
  • Singaravelu Aravinthan Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India.
  • Bethou Adhisivam Department of Pediatrics, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India.
  • Parkash Chand Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), India.

Abstract

Studies have identified the risk factors like folic acid deficiency during gestational period, family history for orofacial clefts, drugs like antiepileptic, vitamin A. But, the data regarding the folic acid status in children with cleft lip/palate is hardly evaluated in depth. Here, an assessment of folic acid and DNA damage were carried out in children with orofacial anomalies. Folic acid level and DNA damage were evaluated by folic acid assay (direct chemiluminescent technology) and single cell gel electrophoresis or comet assay method respectively. The mean value of plasma folic acid by direct chemiluminescent technology was 6.5±3.6 nmol/L and the normal value in children ranges from 11.3 to 47.6 nmol/L. The amount of damaged DNA, measured as the tail length of the comet in cases, was 19.4±8.9 ?m and the mean percentage of DNA in tail was 16.5±3.7. Folic acid deficiency could be the reason for DNA damage.

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Author Biography

Rashmoni Jana, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER)

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Published
2014-03-31
Section
Case Reports
Keywords:
cleft lip, cleft palate, comet assay, orofacial clefts, MTHFR gene.
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How to Cite
Brooklyin, S., Jana, R., Aravinthan, S., Adhisivam, B., & Chand, P. (2014). Assessment of folic acid and DNA damage in cleft lip and cleft palate. Clinics and Practice, 4(1). https://doi.org/10.4081/cp.2014.608