CD34+ stem cells from umbilical cord blood

  • Carlo Pafumi | pafumi@unict.it Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Vito Leanza Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Antonio Carbonaro Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Gianluca Leanza Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Alessandra Iemmola Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Giusi Abate Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Maria Grazia Stracquadanio Gynaecologic and Obstetric Department, University of Catania, Italy.
  • Alfio D’Agati Gynaecologic and Obstetric Department, University of Catania, Italy.

Abstract

We describe the relation between umbilical cord clamping time and two different enrichment system of CD34+ stem cells from umbilical cord blood with the proliferative ability and bone marrow reconstitution of the stem cells obtained. After an obstetrician performed the cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negative selections) using the Stem Sep method, or with a positive cells selection based on their surface CD34 antigens using the Mini Macs system. An excellent recovery of haematopoietic progenitors [Burst Forming Unit Erythroids (BFUE); Colony Forming Unit Granulocytes and Macrophages (CFU-GM); and Colony Forming Unit Granulocytes, Erythroids, Monocytes and Macrophages (CFU-GME)], inversely related to the increase in clamping time, was performed with the Mini Macs system (54% of colonies, with 90% purity). With Stem Sep method, haematopoietic progenitor’s recovery was 35% (with 80% purity). By applying early clamping of umbilical cord blood we obtained a greater number of CD34+ cells and their clonogenic activity was increased with enrichment. This is a useful technique considering that the number of CD34+ stem cells usually contained from a unit of placental blood is enough for the transplant to a child, but not for an adult. Thus, using these methods, we can get a larger number of CD34+ stem cells which reduces the risk of Graft versus Host Disease also in adult patients, producing survival rates similar to those obtained with transplantation of bone marrow from unrelated donors.

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Published
2011-10-27
Section
Articles
Keywords:
CD34 stem cells, umbilical cord blood, recovery, enrichment.
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How to Cite
Pafumi, C., Leanza, V., Carbonaro, A., Leanza, G., Iemmola, A., Abate, G., Stracquadanio, M. G., & D’Agati, A. (2011). CD34+ stem cells from umbilical cord blood. Clinics and Practice, 1(3), e79. https://doi.org/10.4081/cp.2011.e79